Coding

Part:BBa_K2729009:Design

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)


AmCyan_FMDV2a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 820
    Illegal AgeI site found at 669
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AmCyan is optimized for Homo Sapiens in terms of reducing the number of rare codons.


Source

AmCyan is originally isolated from Discosoma coral. FMDV 2A is derived from Foot-and-mouth disease virus.

References